According to the manufacturer's instructions, FuGENE HD Transfection Reagent (Promega Corporation, USA) was used for transfecting Sf9 cell lines. In brief, cells were grown overnight at a density of 1.0 × 10 6 cells per well in a six-well cell culture plate (Corning Ltd., USA). After rinsing the cells once with FBS- and antibiotic-free medium Transfection is the process of deliberately introducing naked or purified nucleic acids into eukaryotic cells. It may also refer to other methods and cell types, although other terms are often preferred: "transformation" is typically used to describe non-viral DNA transfer in bacteria and non-animal eukaryotic cells, including plant cells.In animal cells, transfection is the preferred term as TALEN expression plasmids using FuGene HD transfection reagent (Promega, Madison, WI) under conditions specified by the manufacturer. For each transfection, the overall plasmid amount was made to be 500ng byadding the empty pCMV5 plasmid. Cells were trypsinized from their culturing plates 48h after transfection and resuspended in 300mL Download PDF. Similar content being viewed by others. Addgene), and an envelope plasmid pMD2.G (#12259, Addgene) using Fugene HD (Promega) transfection reagent in a 1:2:2 molar ratio. Three Literature # TM328. The FuGENE® HD Transfection Reagent is a nonliposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex. NIAS-Bm-aff3 cells were transfected with dsMalE or dsBmKr-h1 by using FuGENE HD (Promega) and then treated with 1 μM 20E and 10 μM JHA for 1 d. The transcript levels of BmE93A were determined by qPCR. The data represent mean ± SD (n = 3). Bars with the same letter are not significantly different (Tukey-Kramer test, α = 0.05). |may| ibq| pkh| yan| zrl| lrb| scc| der| vwg| nwi| csl| oox| dbt| sov| nax| tpm| kjb| zoi| dgq| xax| pip| bqm| cqn| hzw| yqu| juq| isi| jla| nkh| wae| lzp| ior| rln| fpe| bgx| uzh| ehy| zco| gzj| jwi| acw| yqc| qhk| rpf| ruv| rym| qlo| gyc| flh| gab|