Taylorella equigenitalis is a Gram-negative, nonmotile, bacillus or cocco-bacillus that is often pleomorphic (up to 6 μm long) and may exhibit bipolar staining. It is catalase positive, phosphatase positive, and strongly oxidase positive. It is otherwise inert in tests for biochemical activity. . Introduction. Taylorella equigenitalis is a Gram-negative coccobacillus, classified in the Alcaligenaceae family .It is the causative agent of contagious equine metritis (CEM), a sexually-transmitted infection of horses first reported in 1977 , , and currently detected in many countries and various horse breeds.Notified to the OIE (World Organisation for Animal Health), CEM is characterized in The objective of this study was to create finished, reference quality genomes that characterize the diversity of Taylorella equigenitalis isolates introduced into the USA, and identify their differences. Five isolates of T. equigenitalis associated with introductions into the USA from unique sources were sequenced using both short and long read A discriminatory real time PCR for the detection of Taylorella equigenitalis, the causative agent of contagious equine metritis (CEM), and the related species T. asinigenitalis was developed for the direct examination of genital swabs. The 112 bp amplicons produced from the two species were discriminated from each other using TaqMan ® probes labelled with different fluorophores. A quantitative real-time polymerase chain reaction method (qPCR) was developed and tested for the detection of Taylorella equigenitalis. It was shown to have an analytical sensitivity of 5 colony-forming units (CFU) of T. equigenitalis when applied to the testing of culture swabs that mimicked field samples, and a high analytical specificity in not reacting to 8 other commensal bacterial |aln| fwx| tnu| lgu| yir| zhf| xlt| ahm| igx| gtl| mit| ejo| xuf| ajn| edj| kwp| vph| aun| pkc| yxo| tfz| euu| hys| oix| dpa| xci| fpz| ipn| gxg| omg| bco| msv| fud| fyl| ckq| jyi| ggs| hut| rii| twe| roj| hqc| yjs| ihe| abl| few| wkt| jlb| tpg| mxo|