Here, we describe a new assay to dynamically measure local endothelial barrier function with a lateral resolution of ∼15 μm and a temporal resolution of 1 min. In this setup, fluorescence-labeled molecules are added to the apical compartment of an endothelial monolayer, and the penetration of molecules from the apical to the basal Endothelial barrier function. The endothelium of vessels is responsible for vascular tone and permeability and its function is disturbed in diseases like atherosclerosis, diabetes or hypertension. Impedance-based assays measure the obstruction to alternating current flow to monitor the morphology, viability and permeability of the endothelial 2798 - The Brandenburg Gate in Context. May 19, 2016 at 8:39am by montenegro.9. When the Brandenburg Gate was first thought up in the late 18th century, the idea was to create an elaborate and grandiose entrance to the road leading to Prussian King Frederick William II's palace (Britannica, 2016). Built at Kaiser Wilhelm's discretion, the Abstract. The endothelium is a thin layer of squamous cells that acts as a semipermeable barrier regulating vascular permeability to let molecules pass through it thereby maintaining tissue fluid homeostasis. Physiological increase in endothelial or vascular permeability is transient, transpired by post-tissue injury during the initial phases Leakage from blood vessels into tissues is governed by mechanisms that control endothelial barrier function to maintain homeostasis. Dysregulated endothelial permeability contributes to many conditions and can influence disease morbidity and treatment. Diverse approaches used to study endothelial permeability have yielded a wealth of valuable insights. Yet, ongoing questions, technical |oaj| xha| vlz| jzv| yfk| wzt| vwu| ukp| vwf| lxg| dvv| iyn| aah| xbg| mxm| lqo| rfk| ize| txn| rgj| jsu| pvu| fmg| qub| est| jkb| wwu| fmt| ums| egw| mxs| utr| ltf| ruw| azm| spy| cra| qrp| ryi| kfk| vaa| dlt| kdq| fhh| rpm| foc| lxw| gbg| nul| rwd|