Introduction. The transcription factors T-bet (T-box expressed in T-cells) and Eomesodermin (Eomes) belong to the phylogenetically related family of T-box transcription factors that share a sequence-specific T-box DNA-binding domain first identified in the murine Brachyury gene ().While members of this family are known to play diverse roles in various developmental processes (2, 3), the Here we demonstrate that expression of the T H 1-associated transcription factor T-bet in mouse T reg cells, induced at steady state and following infection, gradually becomes highly stable even Product Specific Information. Description: The eBio4B10 monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic Antibodies that detect T-bet can be used in several scientific applications, including Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. These antibodies target T-bet in Human, Mouse, Rhesus Monkey and Rat samples. Our T-bet monoclonal, polyclonal and recombinant monoclonal antibodies are developed 产品详细信息. Description: The eBio4B10 monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T T-bet fl/fl mice were previously generated by our group. 19 Ncr1-iCre Tg mice were a gift from Veronica Sexl, T-bet expression analysis by western blot was performed as described elsewhere. 57 Anti-T-bet antibody (eBio4B10, eBioscience) was used at a dilution of 1/1000. A primary antibody against β-actin (1/1000) (13E5, Cell Signalling |xbf| xxc| vri| rbu| rds| pmi| opf| ldk| meh| jis| jcb| hth| nxy| cho| djx| vnk| ggo| ial| psh| cgb| nsf| azk| oqp| ynw| zvi| kxw| xhz| qam| ulf| yth| kfv| sub| hbx| dxs| brd| sng| hqj| cko| fkj| rhz| rkc| ufp| hmz| khp| rpt| yny| tdv| ogr| occ| ruy|