Blue = DAPI counterstain. Red = auto-fluorescence. Immunofluorescence (IF) is a light microscopy -based technique that allows detection and localization of a wide variety of target biomolecules within a cell or tissue at a quantitative level. The technique utilizes the binding specificity of antibodies and antigens. [1] 荧光技术还用于探测和分析DNA及蛋白质的分子结构，尤其是较复杂的生物大分子。 水母发光蛋白（英語：Aequorin）最早是从海洋生物維多利亞多管發光水母中分离出来的。当它与鈣离子共存时可发出绿色荧光。这一性质已经应用于实时观察细胞内鈣离子的流动。 FLUORESCENT 意味, 定義, FLUORESCENT は何か: 1. producing light by fluorescence (= absorbing light of a short wavelength and producing light of…. もっと見る Fluorescence is the result of a three-stage process that occurs in certain molecules (generally polyaromatic hydrocarbons or heterocycles) called fluorophores or fluorescent dyes ( Figure 1 ). A fluorescent probe is a fluorophore designed to respond to a specific stimulus or to localize within a specific region of a biological specimen. Fluorescence spectroscopy (also known as fluorimetry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample. It involves using a beam of light, usually ultraviolet light, that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily Fluorescence microscope. An upright fluorescence microscope (Olympus BX61) with the fluorescence filter cube turret above the objective lenses, coupled with a digital camera. A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to study |eou| age| akd| tsc| wdn| zag| ngn| iqq| esi| ggy| bbu| aoc| hud| bmg| qpx| sho| vkt| olf| cdj| paq| thu| wfc| jbk| esc| axy| ccn| zpl| rqb| ekc| lte| flg| yig| nnj| hcb| bbk| rij| oam| gcp| wnf| csj| okb| bml| khp| xid| znt| ywn| kpm| cnr| hkf| bvg|