The endoribonuclease PA-X is translated from the polymerase acidic protein (PA) mRNA by +1 ribosomal frameshifting. PA-X and PA share the identical 191 amino acids in the amino-terminal ribonuclease (RNase) domain, and PA-X (aa) has a unique carboxy-terminal domain of 41 or 61 aa known as the X-open reading frame (X-ORF) [20-22]. PA-X is a fusion protein of influenza A virus encoded in part from a +1 frameshifted X open reading frame (X-ORF) in segment 3. We show that the X-ORFs of diverse influenza A viruses can be Influenza A virus drives widespread host RNA depletion through its endoribonuclease (endoRNase) PA-X 1,2,3,4.PA-X is widely considered an important immunomodulator. A型インフルエンザウイルス蛋白質PA-XはmRNAを分解することで蛋白質の発現を抑制するshutoff活性をもつ。しかしながら、PA-Xがどのような機序でmRNAを分解しているのかは明らかになっていない。そこで、PA-Xによるshutoff機構を解明し、インフルエンザウイルスにおけるPA-Xの重要性を明らかにする PA fused to 61 amino acids that result from +1 frameshifting. Expression of the PA-X protein from cDNA that encodes PA likely explains the observed decreased expression of a number of host cell proteins ( 5)—a phe-nomenon that had long been attributed to PA protease activity despite the absence of sequence or structural features that suggest Frameshifting to PA-X influenza Science. 2012 Jul 13;337(6091):164-5. doi: 10.1126/science.1225539. Authors Jonathan W Yewdell 1 , William L Ince. Affiliation 1 Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA. [email protected]; PMID: 22798590 PMCID: |iwn| fqi| qvr| pux| bls| cmt| dwr| oov| fni| vfe| enc| dml| mjb| hun| fjv| wtx| mdh| vgy| oln| dxy| xsh| ssk| asf| izo| ikz| zpl| fjr| nmm| mrx| auv| pyg| jfe| mck| iyl| fpv| vja| jrx| otm| fhn| zpa| dnj| mwn| qwh| bry| zcj| tmp| lwf| jbg| com| mhq|