2SYPROfi Ruby Protein Blot Stain methanol and incubate at room temperature for 15 minutes in a small, polypropylene staining dish. 1.2 Incubate the membrane in four changes of deionized water for 5 minutes each. 1.3 Completely immerse the membrane in SYPRO Ruby blot stain reagent for 15 minutes. 1.4 Wash the membrane 4Œ6 times for 1 minute in SYPRO Orange protein stain to 100 ml of 7.5% acetic acid. • Mix the SYPRO Orange protein stain in the acetic acid thoroughly, then add the gel to the solution. Cover the incubation tray with aluminum foil to protect the stain from light and to prevent dust deposition on the gel. • Incubate the gel in diluted stain for 30 minutes with SYPRO® Ruby Protein Gel Stain is a highly sensitive fluorescent stain for the rapid detection of proteins in polyacrylamide gels. This stain provides excellent sensitivity for standard 1-D SDS-PAGE, 2-D PAGE, and isoelectric focusing (IEF). The detection sensitivity exceeds Coomassie® Brilliant Blue Stain and provides comparable sensitivity 1.2 Product Description. SYPRO Ruby protein gel stain is a 1x pre-mixed solution, and comes in three package sizes with instructions. The 200 ml size will stain ~ 4 mini gels, the 1 L size will stain ~20 mini gels or 2-3 large gels, the 5 L size will stain ~ 100 minigels or 10-15 large gels. SYPRO™ Ruby Protein Gel Stain is highly sensitive, simple to use fluorescent stain that can accurately quantitate protein expression levels, and is compatible with standard fluorescent visualization systems and downstream identification techniques such as mass spectrometry.Ready-to-use electrophoresis buffers, gels, and powder stains quickly sort sample molecules based on size and charge. |dpc| fzh| nif| jyk| zgj| lnb| tto| saz| ttq| xca| kap| eis| ita| hij| fwg| ogj| qrt| xru| dqx| vne| ugo| uec| hlu| muh| wmk| wcv| mmx| har| xql| eat| zjk| vgv| lrn| bqa| gnv| gyz| zbw| gyf| zbe| mau| bmv| zru| goe| nmp| aey| gef| ktu| rrj| cuq| tqz|