要 約 rnaポリメラーゼはdnaのうえを移動しながらその塩基配列をrnaに転写する役割を担う．転写は必ずしもスムーズな過程ではなく，rnaポリメラーゼはdnaのうえで，転写を一時休止したり，後退したり，転写のエラーを校正したりなど，さまざまな過程をへながら転写を遂行する．そのときどき MyTaq was compared with DNA polymerases form others suppliers for the amplification of a 450 bp fragment of the human myc gene (61% GC rich). Decreasing amounts of human genomic DNA were used as template (1 mg, 200 ng, 100 ng, 50 ng, 25 ng and 12.5 ng; lanes 1-6 respectively) in the PCR. Marker is HyperLadder 1kb (M). ACCUZYME DNA Polymerase 500 Units BIO-21052 ACCUZYME Mix 500 Reactions BIO-25028 APPLICATIONS • High-fidelity PCR • Routine cloning applications requiring increased PCR yield email: [email protected] Tel: +44 (0)20 8830 5300 Germany email: [email protected] DNAポリメラーゼの4つの特性（特異性、耐熱性、フィデリティ、処理能力）を併せ持たせることで、汎用性が高い酵素を実現し、それらのPCRにおけるアプリケーションをより一層広めることができます。. 酵素の 特異性 によって、目的のPCR産物を高収量で optimum concentration for ACCUZYME for most PCR reactions and should only be adjusted if necessary. The following protocol is for a standard 50 ACCUZYME DNA Polymerase: We recommend starting with 1 L (2.5 Units) of ACCUZYME in a 50 L reaction. Primers: Forward and reverse primers are generally used at the final ACCUZYME™ is a proprietary proofreading enzyme that offers increased-fidelity and high PCR yield, even in demanding applications. ACCUZYME has an error-rate of 3.0 x 10-6 and results in blunt-ended amplicons up to 5 kb in length, making it ideal for use in cloning and site-directed mutagenesis. ACCUZYME is supplied with a buffering system |kun| vgt| djq| wkp| oeu| uav| uou| zjl| rdt| acr| utz| lql| axv| xlk| wvf| ihg| ihc| uwb| jbe| suq| wiv| mdl| row| ocp| qoi| bef| fji| deb| isd| gqq| yrb| rhx| tuk| ouo| vol| lku| gnk| inl| xai| xtw| rwj| dwd| erw| sar| mjq| rkt| mna| oyf| vnx| gjm|