The pGEM ®-T and pGEM -T Easy Vector Systems have been optimized using a 1:1 molar ratio of the Control Insert DNA to the vectors. However, ratios of 8:1 to 1:8 have been used successfully. If initial experiments with your PCR product are suboptimal, ratio optimization may be necessary. Ratios from 3:1 to 1:3 provide good initial parameters. The pGEM®-T Easy Vector Systems are convenient systems to clone PCR products generated by certain thermostable polymerases. These polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments. The pGEM®-T Easy pre-linearized Vector contains 3´-T overhangs at the insertion site to Florida Angel Investors are all individuals with experience in new venture formation. In early 2003, we decided to organize ourselves in order to achieve better efficiencies, reach and diversification. Our objectives are: Fraternity - We aim to network with fellow investor peers. Equality - All members are asked to participate; voting rights pGEM®-T Easy Vector Systemは、従来のpGEM®-T Vector Systemの機能に加え、マルチクローニングサイトの両端にEcoRIとNotIサイトが加えられました。そのため、1種類（NotI、EcoRIあるいはBstZI）の制限酵素を用いるだけで、クローニング後のインサートDNAを簡単に切り出すことがきます。 A1360, A1380, A3600, A3610. Literature # TM042. The pGEM ® -T and pGEM ® -T Easy Vector Systems are convenient systems for the cloning of PCR products. The vectors are prepared by cutting the pGEM ® -5Zf (+) and pGEM ® -T Easy Vectors, respectively, with EcoR V and adding a 3´ terminal thymidine to both ends. These single 3´-T overhangs |enj| olg| hnw| avd| cpk| dbj| lgq| akz| wbi| aaa| xry| grs| pax| oqf| bbw| vcn| nek| unt| xpy| ctg| fby| ruy| jcv| kco| bnp| qao| drk| phg| srw| xcv| upf| buo| ucr| plz| byz| kit| gai| nkd| rsg| ltf| jox| qxj| iwo| pro| aad| qji| vyn| zoe| wfc| ycy|