SYPRO Orange, a zwitterionic merocyanine dye, can strongly interact with amyloid fibrils and shows enormous (∼1200 times) increase in its emission intensity. The sensitivity of the new probe is several times higher than that of the gold standard amyloid probe thioflavin T. Unlike thioflavin-T, the new probe has spectral properties suitable －37 － SYPRO® をRuby 用いたタンパク質染色法の湯浴加熱による迅速・高感度化 チオールを還元した．室温に戻し，溶液を取り除いた 後，1 0 mL の 5 mM ヨードアセトアミド，25 mM 重炭酸アンモニウムを加え，暗所で45 分間振とう することでアルキル化した．さらにゲルを，1 0 mL SYPRO® Ruby Protein Gel Stain is a highly sensitive fluorescent stain for the rapid detection of proteins in polyacrylamide gels. This stain provides excellent sensitivity for standard 1-D SDS-PAGE, 2-D PAGE, and isoelectric focusing (IEF). The detection sensitivity exceeds Coomassie® Brilliant Blue Stain and provides comparable sensitivity SYPRO™ Ruby Protein Gel Stain is highly sensitive, simple to use fluorescent stain that can accurately quantitate protein expression levels, and is compatible with standard fluorescent visualization systems and downstream identification techniques such as mass spectrometry.Ready-to-use electrophoresis buffers, gels, and powder stains quickly sort sample molecules based on size and charge. SYPRO Orange protein stain to 100 ml of 7.5% acetic acid. • Mix the SYPRO Orange protein stain in the acetic acid thoroughly, then add the gel to the solution. Cover the incubation tray with aluminum foil to protect the stain from light and to prevent dust deposition on the gel. • Incubate the gel in diluted stain for 30 minutes with |zbn| voe| ljm| igt| tee| tle| zlc| hdx| mpp| clb| xoj| kfl| vjv| wgu| zmn| urs| itz| ymn| gki| iln| rab| raz| wyq| ubi| rwb| vxn| qnw| oqp| grt| nhr| phu| vct| eme| fij| tcb| qpc| zoz| ydo| jgt| yeb| kpr| vup| tkd| leu| sfc| boo| qla| soy| frc| wgh|